Prostaglandin E Synthase (PTGES) Knockout

file:///Users/hyoung-soocho/Downloads/ch8f33.jpg Divangahi et al. Eicosanoid pathways regulate adaptive immunity to Mycobacterium tuberculosis.Nature immunology, Vol. 11, No. 8. (11 August 2010), pp. 751-758

1. Hyoung-Soo: How was the Ptges -/- mouse made? Is the entire gene deleted or only part?

 Prostaglandin E Synthase Knockout Mice (Ptges-/-) 

Mouse description

Mice homozygous for this Ptges2 (prostaglandin E synthase 2) targeted mutation exhibit reduced prostaglandin E2 synthesis

Availability: Under development for distribution colony (JAX mice)'

Creator: Beverly Koller (UNC-CH)

Detailed information was not given on the creation of this allele.

Targeting gene: mPGES2 (Ptges2tm1bhk) Prostaglandin synthase 2 gene disruption causes severe renal pathology in the mouse. Cell. 83(3):473-82 (1995)

A targeting vector containing neomycin resistance (NeoR) and herpes simplex virus thymidine kinase (TK) genes was used to disrupt exon 4.

Selection markers for electroporated ES cells

1) Neomycin resistance gene: G-418 (Positive selection)

2) Thymidine kinase gene: Gangcylovir (Negative selection)

Reference

1. Chen, M. et al. Lipid mediators in innate immunity against tuberculosis: opposing roles of PGE2 and LXA4 in the induction of macrophage death. J. Exp. Med. 205, 2791-801 (2008)

2. Tilley, S.L. et al. Reproductive failure and reduced blood pressure in mice lacking the EP2 prostaglandin E2 receptor. J. Clin. Invest. 103:1539-154 (1999)

3. Khandrasekharan S et al. Coupling of COX-1 to mPGES1 for prostaglandin E2 biosynthesis in the murine mammary gland. J Lipid Res 46(12):2636-48 (2005)

4. Prostaglandin synthase 2 gene disruption causes severe renal pathology in the mouse. Cell. 83(3):473-82 (1995)

5. JAX mice for mouse information

http://jaxmice.jax.org/strain/009135.html

6. Knock-out mice generation procedure scheme picture

http://linguamedica.jp/mita/20030618/knockout/knockout.htm